必须声明标量变量 "@Script_ID"。 茶枯饼提取物对骨髓来源巨噬细胞炎症反应的作用研究-《赣南医学院学报》

[1]王 斐,何伟亮,谢 璐,等.茶枯饼提取物对骨髓来源巨噬细胞炎症反应的作用研究[J].赣南医学院学报,2019,39(10):979-983.[doi:10.3969/j.issn.1001-5779.2019.10.002]
 WANG Fei,HE Wei-liang,XIE Lu,et al.Effect of camellia cake extracts on inflammatory response of bone marrow-derived macrophages[J].,2019,39(10):979-983.[doi:10.3969/j.issn.1001-5779.2019.10.002]
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茶枯饼提取物对骨髓来源巨噬细胞炎症反应的作用研究()
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《赣南医学院学报》[ISSN:1001-5779/CN:36-1154/R]

卷:
39
期数:
2019年10期
页码:
979-983
栏目:
炎症与免疫·基础与临床
出版日期:
2019-11-24

文章信息/Info

Title:
Effect of camellia cake extracts on inflammatory response of bone marrow-derived macrophages
文章编号:
1001-5779(2019)10-0979-05
作者:
王 斐1何伟亮2谢 璐3曾招林4陈水亲3宋 涛3刘志平3
赣南医学院1.2017级硕士研究生; 2.2016级生物技术本科生; 3.基础医学院; 4.第一附属医院,江西 赣州 341000
Author(s):
WANG Fei1 HE Wei-liang2 XIE Lu3 ZENG Zhao-lin4 CHEN Shui-qin3 SONG Tao3 LIU Zhi-ping3
Gannan Medical University 1.Grade 2017 Graduate Student; 2.Grade 2016 Biotechnology Undergraduate; 3.School of Basic Medicine; 4.The First Affiliated Hospital, Ganzhou, Jiangxi 341000
关键词:
茶枯饼提取物 巨噬细胞 炎症细胞因子
Keywords:
Camellia cake extracts Macrophages Inflammatory cytokines
分类号:
R285.5
DOI:
10.3969/j.issn.1001-5779.2019.10.002
文献标志码:
A
摘要:
目的:研究茶枯饼提取物(CCEs)对脂多糖(LPS)诱导的小鼠骨髓来源巨噬细胞(BMDM)细胞炎症反应的作用。方法:培养BMDM后,给予不同浓度的CCEs处理12 h,再利用LPS刺激BMDM细胞,最后用PCR方法测定相关炎性细胞因子的表达水平。结果:LPS刺激BMDM 2~4 h引起炎症细胞因子表达的差异有统计学意义(P<0.05)。与LPS组对照,各浓度的CCEs处理的BMDM细胞在LPS刺激后相关经典炎症细胞因子,主要有白细胞介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)、角质细胞趋化因子(KC)和单核细胞趋化蛋白1(MCP-1)的表达水平差异无统计学意义(P>0.05)。结论:CCEs在目前LPS刺激的BMDM细胞的条件下没有显示抗炎症效应。
Abstract:
Objective: To study the effect of Camellia cake extracts(CCEs)on the inflammatory response of mouse bone marrow-derived macrophages(BMDM)stimulated by lipopolysaccharide(LPS).Methods: BMDM cells were treated by CCEs at different concentrations for 12 hours, then stimulated by LPS.After that, the expression levels of relevant inflammatory cytokines were determined by PCR.Results: LPS stimulation of BMDM for 2-4 hours resulted in a significant increase(P<0.05)in the expression of inflammatory cytokines.Compared to LPS group, there was no significant difference(P>0.05)in the expression of inflammatory cytokines, including IL-6, TNF-α, KC, and MCP-1, in BMDM cells treated with CCEs at different concentrations after LPS stimulation.Conclusions: CCEs have no anti-inflammatory effect on BMDM cells stimulated by LPS under current experimental conditions.

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备注/Memo

备注/Memo:
基金项目:国家自然科学基金项目(31560260); 江西省教育厅科研项目(GJJ170872, GJJ180802); 赣南医学院人才启动基金项目(QD201404); 赣南油茶产业开发协同创新中心开放基金项目(YK201603,YK201615); 赣南医学院校级油茶专项课题(YC201501); 赣南医学院2018年度研究生创新课题(YC2018-X003)
通信作者:刘志平,男,博士,教授,研究方向:炎症和癌症的免疫学机制。E-mail:Zhiping.Liu@gmu.edu.cn
更新日期/Last Update: 2019-11-20