必须声明标量变量 "@Script_ID"。 MuRF1在妊娠生理性肥厚心肌细胞中的表达-《赣南医学院学报》

[1]谢家和ab,周世菊c,王雅兰c,等.MuRF1在妊娠生理性肥厚心肌细胞中的表达[J].赣南医学院学报,2019,39(12):1193-1196+121219.[doi:10.3969/j.issn.1001-5779.2019.12.002]
 XIE Jia-heab,ZHOU Shi-juc,WANG Ya-lanc,et al.Expression of MuRF1 in physiological Myocardial hypertrophy induced by pregnancy[J].,2019,39(12):1193-1196+121219.[doi:10.3969/j.issn.1001-5779.2019.12.002]
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MuRF1在妊娠生理性肥厚心肌细胞中的表达()
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《赣南医学院学报》[ISSN:1001-5779/CN:36-1154/R]

卷:
39
期数:
2019年12期
页码:
1193-1196+121219
栏目:
心脑血管疾病·基础与临床
出版日期:
2019-12-31

文章信息/Info

Title:
Expression of MuRF1 in physiological Myocardial hypertrophy induced by pregnancy
文章编号:
1001-5779(2019)12-1193-05
作者:
谢家和1a1b周世菊1c王雅兰1c廖 沁1c王东泰1d吕亮亮1c陆伟灵1d丁荣明1e廖禹林2
1.赣南医学院 a.第一附属医院心内科; b.心脑血管疾病防治教育部重点实验室; c.2019级硕士研究生; d.2017级硕士研究生; e.2018级硕士研究生,江西 赣州 341000; 2.南方医院心内科,广东 广州 510515
Author(s):
XIE Jia-he1a1b ZHOU Shi-ju1c WANG Ya-lan1c LIAO Qin1c WANG Dong-tai1d LV Liang-liang1c LU Wei-ling1d DING Rong-ming1e LIAO Yu-lin2
1.Gannan Medical University a.The First Affiliated Hospital; b.Key Laboratory of Prevention and Treatment of Cardiovascular and Cerebrovascular Diseases, Ministry of Education; c.Undergraduate student, Grade 2019; d.Postgraduate student, Grade 2017; e.Postgraduate student, Grade 2018, Ganzhou, Jiangxi 341000; 2.Department of Cardiology, Nanfang Hospital, Guangdong Guangzhou 510515
关键词:
肌肉环状指基因1 妊娠 心肌肥厚
Keywords:
muscle ring finger 1 pregnancy myocardial hypertrophy
分类号:
R322.1+1
DOI:
10.3969/j.issn.1001-5779.2019.12.002
文献标志码:
A
摘要:
目的:检测肌肉环状指基因1(MuRF1)在妊娠生理性肥厚心肌细胞中的表达及观察其对妊娠肥厚心肌细胞功能可能的影响。方法:比较未经历妊娠雌性小鼠(对照组),同鼠龄妊娠18~20天小鼠(妊娠组)心重(HW),心重/胫骨长度比(HW/TL)及细胞面积大小。使用qPCR及Western Blot技术检测两组小鼠心肌病理性心肌肥厚标志物Nppa、Myh7和MuRF1表达。结果:与对照组相比,妊娠组小鼠心脏增大、心肌细胞面积增加。两组小鼠心肌细胞的Nppa及Myh7表达没有差异,而MuRF1在妊娠生理性心肌肥厚细胞中低表达。结论:妊娠可诱导母体心肌生理性肥厚,并抑制母体心肌细胞MuRF1的表达,提示MuRF1异常可能与妊娠相关心脏疾病发生有密切联系。
Abstract:
Objective: To test the expression of muscle ring finger gene 1(MuRF1)in hypertrophic cardiomyocytes induced by pregnancy and further infer its potential effect on pregnancy related heart disease. Methods: Heart weight, heart weight/tibia length ratio(HW/TL)and cardiomyocyte areas were compared between the control group( virgin mice )and the pregnant group(18-20 days of pregnant mice). The molecular techniques of qPCR and western blot were employed to test the expression of pathological myocardial hypertrophic makers(Nppa, Myh7)and MuRF1. Results: Compared with the control group, Heart weight, heart weight / tibia length ratio(HW/TL)and cardiomyocyte areas were increased in pregnant group. There were no difference in Nppa, Myh7 between two groups and the reduced MuRF1 was in pregnant group. Conclusions: pregnancy induced myocardial physiological hypertrophy, and the low expression of MuRF1 in hypertrophic cardiomyocytes induced by pregnancy, which indicated the abnormal expression of MuRF1 may be closely related to pregnancy-related heart diseases.

参考文献/References:

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备注/Memo

备注/Memo:
基金项目:国家自然科学基金项目(编号:81960349); 江西省教育厅一般项目(编号:GJJ180813); 赣南医学院校级科研项目(ZD201826)
作者简介:谢家和,男,博士,副主任医师,主要从事心血管疾病研究。E-mail:xiejiahezg@126.com
更新日期/Last Update: 2019-12-30