必须声明标量变量 "@Script_ID"。 人参皂苷Rg1、Rb1抗Aβ人血管内皮细胞损害作用的研究-《赣南医学院学报》

[1]刘南海a,黄晓峰b,王振凤c,等.人参皂苷Rg1、Rb1抗Aβ人血管内皮细胞损害作用的研究[J].赣南医学院学报,2019,39(12):1197-1200+1240.[doi:10.3969/j.issn.1001-5779.2019.12.003]
 LIU Nan-haia,HUANG Xiao-fengb,WANG Zhen-fengc,et al.Study on the effect of ginsenoside Rg1 and Rb1 for the anti-damage of Aβ on human vascular endothelial cells[J].,2019,39(12):1197-1200+1240.[doi:10.3969/j.issn.1001-5779.2019.12.003]
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人参皂苷Rg1、Rb1抗Aβ人血管内皮细胞损害作用的研究()
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《赣南医学院学报》[ISSN:1001-5779/CN:36-1154/R]

卷:
39
期数:
2019年12期
页码:
1197-1200+1240
栏目:
心脑血管疾病·基础与临床
出版日期:
2019-12-31

文章信息/Info

Title:
Study on the effect of ginsenoside Rg1 and Rb1 for the anti-damage of Aβ on human vascular endothelial cells
文章编号:
1001-5779(2019)12-1197-05
作者:
刘南海1a黄晓峰1b王振凤1c章 健2符雪涛3薛寿儒3朱长东4杨 凯1d罗江洪1e
1.赣南医学院 a.第一附属医院; b.护理学院; c.第二附属医院; d.药学院; e.基础医学院,江西 赣州 341000; 2.南昌县人民医院,江西 南昌 330000; 3.苏州大学附属第一医院,江苏 苏州 215000; 4.赣州市立医院,江西 赣州 341000
Author(s):
LIU Nan-hai1a HUANG Xiao-feng1b WANG Zhen-feng1c ZHANG Jian2 FU Xue-tao3 XUE Shou-ru3 ZHU Chang-dong4 YANG Kai1d LUO Jiang-hong1e
1.Gannan Medical University a.The First Affiliated Hospital; b.Nursing College; c.The Second Affiliated Hospital; d.pharmacy college; e.Basic Medical College, Ganzhou, Jiangxi 341000; 2.Nanchang County People's Hospital, Nanchang, Jiangxi 330000; 3.The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215000; 4.Ganzhou Municipal Hospital, Ganzhou, Jiangxi 341000
关键词:
人血管内皮细胞 β-淀粉样蛋白 人参皂苷 增殖
Keywords:
Human vascular endothelial cell β-amyloid protein Ginsenoside Proliferation
分类号:
R329.2+8
DOI:
10.3969/j.issn.1001-5779.2019.12.003
文献标志码:
A
摘要:
目的:探讨人参皂苷Rg1、Rb1对β-淀粉样蛋白(Aβ)诱导人脑血管内皮细胞增殖抑制情况的影响。方法:将人脑血管内皮细胞分为对照组、Aβ1-42组(20 μmol·L-1)、低人参皂苷Rg1组(20 μmol·L-11-42+Rg1 0.1 μmol·L-1)、中人参皂苷Rg1组(20 μmol·L-11-42+Rg1 1 μmol·L-1)、高人参皂苷Rg1组(20 μmol·L-11-42+Rg1 10 μmol·L-1)、低人参皂苷Rb1组(20 μmol·L-11-42+Rb1 0.1 μmol·L-1)、中人参皂苷Rb1组(20 μmol·L-11-42+Rb1 1 μmol·L-1)、高人参皂苷Rb1组(20 μmol·L-11-42+Rb1 10 μmol·L-1)。用MTT检测各组细胞增殖能力,Western-blot检测各组细胞中PCNA、Ki67、COX-2蛋白表达情况,ELISA法检测各组细胞中IL-1、TNF-α水平。结果:与对照组相比,Aβ1-42组细胞的增殖率、PCNA、Ki67蛋白表达显著降低(P<0.05),COX-2、IL-1、TNF-α水平显著升高,与Aβ1-42组细胞相比,低、中、高人参皂苷Rg1和低、中、高人参皂苷Rb1组细胞的增殖率、PCNA、Ki67蛋白表达显著升高(P<0.05),COX-2、IL-1、TNF-α水平显著降低(P<0.05)。结论:人参皂苷Rg1、Rb1可能是通过促进增殖蛋白及抑制炎症因子表达来改善Aβ导致的人脑血管内皮细胞增殖抑制。
Abstract:
Objective: To explore effects of ginsenoside Rg1 and Rb1 on proliferation inhibition of β-amyloid protein(Aβ)-induced human brain microvascular endothelial cells. Methods: Human brain microvascular endothelial cells were divided into control group, Aβ1-42 group(20 μmol?L-1), low ginsenoside Rg1 group(20 μmol?L-11-42+Rg1 0.1 μmol?L-1), medium ginsenoside Rg1 group(20 μmol?L-11-42+Rg1 1 μmol?L-1), high ginsenoside Rg1 group(20 μmol?L-11-42+Rg1 10 μmol?L-1), low ginsenoside Rb1 group(20 μmol?L-11-42+Rb1 0.1 μmol?L-1), medium insenoside Rb1 group(20 μmol?L-11-42+Rb1 1 μmol?L-1), and high ginsenoside Rb1 group(20 μmol?L-11-42+Rb1 10 μmol?L-1). The cell proliferation ability in each group was detected by MTT. The expression of proliferating PCNA, Ki67 and COX-2 protein in each group was detected by Western-blot. The levels of IL-1 and TNF-α in each group were detected by ELISA. Results: Compared with control group, proliferation rate, expression of PCNA and Ki67 protein in Aβ1-42 group were significantly decreased(P<0.05), while levels of COX-2, IL-1 and TNF-α were significantly increased. Compared with Aβ1-42 group, proliferation rate, expression of PCNA and Ki67 protein in low, medium, high ginsenoside Rg1 group, and low, medium, high ginsenoside Rb1 group were significantly increased(P<0.05), while levels of COX-2, IL-1 and TNF-α were significantly decreased(P<0.05). Conclusions: Ginsenoside Rg1 and Rb1 may improve proliferation inhibition of human brain microvascular endothelial cells induced by Aβ in the way of promoting proliferation proteins and inhibiting expression of inflammatory factors.

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备注/Memo

备注/Memo:
基金项目:江西省教育厅科学技术研究项目(GJJ150960); 赣南医学院产学研项目(CP201407)
通信作者:薛寿儒,男,教授,主任医师,博士生导师,研究方向:神经变性病和痴呆基础与临床研究。E-mail:xueshouru@suda.edu.cn
更新日期/Last Update: 2019-12-30